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rab7 d95f2 (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc rab7 d95f2

(A) Representative confocal immunofluorescence microscopy images of Rab5, EEA1, <t>Rab7,</t> CD63 and LAMP1 (red) recruited or not to the ABC141 (yellow) ACVs in EA.hy926 endothelial cells at 10, 20, 30 min or 1, 2, 6 and 24h. DAPI was used to visualize nuclei (blue) and bacteria. (B) Quantification of the percentage of ACVs positive for V-ATPase at different time points after infection. Data correspond to the means ± SD of 3 independent experiments. (C) Representative images of V-ATPase positive (red) ABC141 (white) ACVs at 30 min (left) and 24h post-infection (right). DAPI was used to visualize nuclei and bacteria. All scale bars correspond to 5 μm.

Rab7 D95f2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/d95f2/pmc12912691-74-1-8
Average 86 stars, based on 1 article reviews

rab7 d95f2 - by Bioz Stars, 2026-09

86/100 stars

Images

1) Product Images from "Differential roles of the type I and II secretion systems for the intracellular ABC141 Acinetobacter baumannii infection, which elicits an atypical hypoxia response in endothelial cells"

Article Title: Differential roles of the type I and II secretion systems for the intracellular ABC141 Acinetobacter baumannii infection, which elicits an atypical hypoxia response in endothelial cells

Journal: PLOS Pathogens

doi: 10.1371/journal.ppat.1013265

(A) Representative confocal immunofluorescence microscopy images of Rab5, EEA1, Rab7, CD63 and LAMP1 (red) recruited or not to the ABC141 (yellow) ACVs in EA.hy926 endothelial cells at 10, 20, 30 min or 1, 2, 6 and 24h. DAPI was used to visualize nuclei (blue) and bacteria. (B) Quantification of the percentage of ACVs positive for V-ATPase at different time points after infection. Data correspond to the means ± SD of 3 independent experiments. (C) Representative images of V-ATPase positive (red) ABC141 (white) ACVs at 30 min (left) and 24h post-infection (right). DAPI was used to visualize nuclei and bacteria. All scale bars correspond to 5 μm.

Figure Legend Snippet: (A) Representative confocal immunofluorescence microscopy images of Rab5, EEA1, Rab7, CD63 and LAMP1 (red) recruited or not to the ABC141 (yellow) ACVs in EA.hy926 endothelial cells at 10, 20, 30 min or 1, 2, 6 and 24h. DAPI was used to visualize nuclei (blue) and bacteria. (B) Quantification of the percentage of ACVs positive for V-ATPase at different time points after infection. Data correspond to the means ± SD of 3 independent experiments. (C) Representative images of V-ATPase positive (red) ABC141 (white) ACVs at 30 min (left) and 24h post-infection (right). DAPI was used to visualize nuclei and bacteria. All scale bars correspond to 5 μm.

Techniques Used: Immunofluorescence, Microscopy, Bacteria, Infection

Clone Assay:

Article Title: A conserved role for the ESCRT membrane budding complex in LINE retrotransposition
Article Snippet: .. Commercially available antibodies used: anti-HA affinity matrix (Sigma #11815016001), mouse anti-HA clone 12CA5 (Sigma #11583816001), rat anti-HA clones 3F10 (Sigma #11867423001), rabbit polyclonal anti-ALIX (Bethyl #A302-938A), mouse monoclonal anti-CHMP3 clone F-1 (Santa Cruz #sc-166361), rabbit polyclonal anti-CHMP6 clone FL-201 (Santa Cruz #sc-67231), rabbit polyclonal anti-H3 (Abcam #ab1791), mouse monoclonal anti-γ-H2AX clone JBW301 (EMD Millipore #05-636-I), mouse monoclonal anti-ICAM-1, clone 15.2 (Santa Cruz #sc-107), mouse monoclonal anti-tubulin clone DM1A (Sigma #T6199), rabbit monoclonal anti-EEA1 clone C45B10 (Cell Signaling Technology #3288), rabbit monoclonal anti-Rab7 clone D95F2 (Cell Signaling Technology #9367), mouse monoclonal anti-LAMP2 clone H4B4 (Abcam #ab25631), mouse monoclonal anti-calnexin clone AF18 (ThermoFisher #MA3-027), rabbit monoclonal anti-AIF clone D39D2 (Cell Signaling Technology #5318), mouse monoclonal anti-RCAS1 clone D9 (Santa Cruz #sc-398052), rabbit polyclonal anti-EDC4 (Cell Signaling Technology #2548), rabbit monoclonal anti-eIF3H clone DC91 (Cell Signaling Technology #3413). ..

other:

Article Title: Synthetic Lethal Interaction between the ESCRT Paralog Enzymes VPS4A and VPS4B in Cancers Harboring Loss of Chromosome 18q or 16q
Article Snippet: RAB7 , Rabbit , Clone D95F2 , , Cell Signaling Technology , 1 , #9367S.

Staining:

Article Title: Assessment of Ocriplasmin Effects on the Vitreoretinal Compartment in Porcine and Human Model Systems
Article Snippet: .. After treatment, cells were washed, fixed in 4% PFA, and stained with indicated antibodies: Rab7 clone D95F2, Rab11 clone D4F5 (Cell Signaling Technologies), and goat anti-rabbit Alexa 555 (Life technologies) within 48 h. Cells were imaged via epifluorescence (Zeiss Axio Imager 2) as well as confocal microscopy (Nikon C2+). .. For flow cytometry, cells were washed with PBS after treatment and detached with Accutase (Sigma-Aldrich).

Confocal Microscopy:

Immunohistochemistry:

Article Title: Mitotic regulators and the SHP2-MAPK pathway promote IR endocytosis and feedback regulation of insulin signaling
Article Snippet: .. The following antibodies were purchased from commercial sources: anti-ZO-1/TJP1 (Immunohistochemistry (IHC), 1:100; #402200) and anti-ACTIN (Western blotting (WB), 1:1000; MA137018, Thermo Scientific); anti-IR-pY1150/1151 (WB, 1:1000; 19H7; labeled as pY1152/1153 IR for mouse IR in this study), anti-IRS1-pS616 (WB, 1:1000; C15H5; labeled as pS612 IRS1 for mouse IRS1 in this study), anti-AKT (WB, 1:1000; 40D4), anti-pT308 AKT (WB, 1:1000; D25E6), anti-ERK1/2 (WB, 1:1000; L34F12), anti-pERK1/2 (WB, 1:1000; 197G2), anti-SHP2 (WB, 1:1000; D50F2), and anti-RAB7 (Immunofluorescence (IF), 1:200; D95F2, Cell Signaling); anti-IRS1-pY612 (WB, 1:1000; labeled as pY608 IRS1 for mouse IRS1 in this study), anti-IR-pY972 (WB, 1:1000; labeled as pY962 IR for mouse IR in this study), and anti-IR (WB, 1:1000; IF, 1:100; CT-3, Millipore); anti-AP2B1 (WB, 1:1000; BD Biosciences); anti-IRS2 (WB, 1:1000; EPR904) and anti-AP2M1 (WB, 1:1000; EP2695Y, Abcam); anti-GFP (IF, 1:500; #11814460001) and anti-MYC (WB, 1:1000; IF, 1:500; 9E10; Roche); anti-IRS1 (WB, 1:1000; A301-158A, Bethyl Laboratory); anti-CEACAM1 (WB, 1:1000; 283340, R&D Systems); anti-ACTIN (WB, 1:1000; C-4), anti-IR (WB, 1:1000; IF, 1:100; CT-3, Santa Cruz); and anti-mCherry (IF, 1:100; 1C51, Novus). .. The siRNAs were synthesized by Dharmacon (Lafayette, CO) and had the following sequences: human BUBR1 (GGACACAUUUAGAUGCACUtt); human CDC20 (AGAACAGACUGAAAGUACUUU); human MAD1 (GAGCAGAUCCGUUCGAAGUUU); human BUB3 (GAGUGGCAGUUGAGUAUUU); human BUB1 (GAGUGAUCACGAUUUCUAA); human IRS1 (GAACCUGAUUGGUAUCUACdTdT); human IRS2 (On-TARGETplus human IRS2 (8660) siRNA-SMARTpool); human CEACAM1 #1 (CCAUCAUGCUGAACGUAAA); human CEACAM1 #2 (GAUCAUAGUCACUGAGCUA); human CEACAM1 #3 (CGUAUUGGUGUGAGGUCUU); human CEACAM1 #4 (CCAUUAAGUACAUGUGCCA); human IR (GGGUGGAAGUUUAGGGUUAUU); and siLUC (UCAUUCCGGAUACUGCGAU).

Image Search Results

Journal: PLOS Pathogens

doi: 10.1371/journal.ppat.1013265

Figure Lengend Snippet: (A) Representative confocal immunofluorescence microscopy images of Rab5, EEA1, Rab7, CD63 and LAMP1 (red) recruited or not to the ABC141 (yellow) ACVs in EA.hy926 endothelial cells at 10, 20, 30 min or 1, 2, 6 and 24h. DAPI was used to visualize nuclei (blue) and bacteria. (B) Quantification of the percentage of ACVs positive for V-ATPase at different time points after infection. Data correspond to the means ± SD of 3 independent experiments. (C) Representative images of V-ATPase positive (red) ABC141 (white) ACVs at 30 min (left) and 24h post-infection (right). DAPI was used to visualize nuclei and bacteria. All scale bars correspond to 5 μm.

Article Snippet: , Rab7 (D95F2) , Rabbit , 1:200 , Cell signaling 9367T.

Techniques: Immunofluorescence, Microscopy, Bacteria, Infection

RSV entry into cells triggers actin polymerization and endosome formation by cholesterol-rich lipid rafts. ( A ) RSV enters cells by actin-mediated endocytosis. HEp-2 or A549 cells were infected with RSV A2 (MOI = 30) (90 min, 37°C) and fixed (4% PFA, 15 min, room temperature). Cell nuclei were labeled with DAPI (cyan), lipid rafts with Alexa 647-CTB (blue), cholesterol with NBD-cholesterol (green), and F-actin with CellMask orange actin tracking dye (yellow), and images were acquired using confocal microscopy. Scale bar = 10 µm. Intensity profiles show colocalization along the direction of the yellow line. Pearson’s correlation between lipid rafts, cholesterol or F-actin, and RSV. n = 16 cells per group. Error bars represent a 95% confidence interval. ( B ) RSV trafficking from early to late endosomes. HEp-2 or A549 cells were infected with RSV A2 (MOI = 30) (150 min, 37°C) and fixed. Cell nuclei were labeled with DAPI (cyan), lipid rafts with Alexa 647-CTB (blue), and cholesterol with NBD-cholesterol (green). Immunodetection of RSV F (blue), RSV-FITC (green), EEA1, Rab7, and STX 6 (red) was performed using confocal microscopy. Scale bar = 10 µm. Intensity profiles show colocalization along the direction of the yellow line. Pearson’s correlation between lipid rafts, cholesterol or F-actin, and RSV. n = 16 cells per group. Error bars represent a 95% confidence interval.

Journal: Microbiology Spectrum

Article Title: Cholesterol-rich lipid rafts mediate endocytosis as a common pathway for respiratory syncytial virus entry into different host cells

doi: 10.1128/spectrum.01192-25

Figure Lengend Snippet: RSV entry into cells triggers actin polymerization and endosome formation by cholesterol-rich lipid rafts. ( A ) RSV enters cells by actin-mediated endocytosis. HEp-2 or A549 cells were infected with RSV A2 (MOI = 30) (90 min, 37°C) and fixed (4% PFA, 15 min, room temperature). Cell nuclei were labeled with DAPI (cyan), lipid rafts with Alexa 647-CTB (blue), cholesterol with NBD-cholesterol (green), and F-actin with CellMask orange actin tracking dye (yellow), and images were acquired using confocal microscopy. Scale bar = 10 µm. Intensity profiles show colocalization along the direction of the yellow line. Pearson’s correlation between lipid rafts, cholesterol or F-actin, and RSV. n = 16 cells per group. Error bars represent a 95% confidence interval. ( B ) RSV trafficking from early to late endosomes. HEp-2 or A549 cells were infected with RSV A2 (MOI = 30) (150 min, 37°C) and fixed. Cell nuclei were labeled with DAPI (cyan), lipid rafts with Alexa 647-CTB (blue), and cholesterol with NBD-cholesterol (green). Immunodetection of RSV F (blue), RSV-FITC (green), EEA1, Rab7, and STX 6 (red) was performed using confocal microscopy. Scale bar = 10 µm. Intensity profiles show colocalization along the direction of the yellow line. Pearson’s correlation between lipid rafts, cholesterol or F-actin, and RSV. n = 16 cells per group. Error bars represent a 95% confidence interval.

Article Snippet: The Flotillin-1 (D2V7J) rabbit mAb (#18,934T), caveolin-1 (D46G3) rabbit mAb (#3267), EEA1 (C45B10) rabbit mAb (#3288), Rab7 (D95F2) rabbit mAb (#9367), Rab7 (E9O7E) mouse mAb (#95746), and syntaxin 6 (C34B2) rabbit mAb (#2869) were purchased from Cell Signaling Technology.

Techniques: Infection, Labeling, Confocal Microscopy, Immunodetection

Interaction and colocalization of the PEDV S protein with IFITM proteins. ( A ) Co-IP assays were performed to assess the interaction between the PEDV S1 protein and human or porcine IFITM proteins. HEK293T cells were cotransfected with plasmids encoding PEDV S1-Fc and various HA-tagged IFITM proteins. At 24 h post-transfection, the cells were harvested. Immunoprecipitation was conducted using an Fc tag antibody, and the presence of IFITM proteins was detected by Western blotting (IB: Anti-HA). Whole-cell lysates (WCLs) were analyzed to confirm expression levels (IB: Anti-Fc, Anti-HA, Anti-β-Tubulin). The Co-IP was repeated three times, yielding similar results. ( B ) Confocal microscopy images showing the intracellular localization of porcine IFITM1 in LLC-PK1 cells are presented. IFITM1 (red) colocalizes with clathrin (green), EEA1 (green), Rab7 (green), and LAMP7 (green), as indicated in the merged images. DAPI (blue) was used to stain the nuclei. ( C ) Confocal microscopy images demonstrating the colocalization of the PEDV S protein (red) with HA-IFITM1 (green) in LLC-PK1 cells are shown. The merged image shows the nuclei stained with DAPI (blue).

Journal: Journal of Virology

Article Title: IFITM proteins are key entry factors for porcine epidemic diarrhea coronavirus

doi: 10.1128/jvi.02028-24

Figure Lengend Snippet: Interaction and colocalization of the PEDV S protein with IFITM proteins. ( A ) Co-IP assays were performed to assess the interaction between the PEDV S1 protein and human or porcine IFITM proteins. HEK293T cells were cotransfected with plasmids encoding PEDV S1-Fc and various HA-tagged IFITM proteins. At 24 h post-transfection, the cells were harvested. Immunoprecipitation was conducted using an Fc tag antibody, and the presence of IFITM proteins was detected by Western blotting (IB: Anti-HA). Whole-cell lysates (WCLs) were analyzed to confirm expression levels (IB: Anti-Fc, Anti-HA, Anti-β-Tubulin). The Co-IP was repeated three times, yielding similar results. ( B ) Confocal microscopy images showing the intracellular localization of porcine IFITM1 in LLC-PK1 cells are presented. IFITM1 (red) colocalizes with clathrin (green), EEA1 (green), Rab7 (green), and LAMP7 (green), as indicated in the merged images. DAPI (blue) was used to stain the nuclei. ( C ) Confocal microscopy images demonstrating the colocalization of the PEDV S protein (red) with HA-IFITM1 (green) in LLC-PK1 cells are shown. The merged image shows the nuclei stained with DAPI (blue).

Article Snippet: Additional antibodies included EEA1 mouse monoclonal antibody (cat#: 68065-1-Ig), HA tag mouse monoclonal antibody (cat#: 66006-2-Ig), Rab7 (D95F2) XP rabbit monoclonal antibody (cat#: 9367s), and CLTC monoclonal antibody (cat#: 66487-1-Ig) from Proteintech.

Techniques: Co-Immunoprecipitation Assay, Transfection, Immunoprecipitation, Western Blot, Expressing, Confocal Microscopy, Staining